Nuova pubblicazione - "Determinazione della struttura polisaccaridica capsulare del ceppo rappresentativo KPB-1 di Klebsiella pneumoniae ST512 e assegnazioni delle funzioni glicosiltransferasi"

Pubblicato sulla rivista International Journal of Biological Macromolecules dal gruppo di ricerca della dott.ssa Paola Cescutti
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Klebsiella pneumoniae strain KPB-1 was isolated in early 2011 from the pleural fluid of an inpatient admitted at an Italian hospital. It was characterized to produce the KPC-3 carbapenemase and to belong to sequence type 512, a derivative of sequence type 258 clade II characterized by the cps-2 gene cluster. The K-antigen of K. pneumoniae KPB-1 was purified and its structure determined by using GLC-MS of appropriate carbohydrate derivatives and 1D and 2D NMR spectroscopy of the native polysaccharide. All the collected data demonstrated the following repeating unit for the K. pneumoniae KPB-1 capsular polysaccharide: The reactions catalysed by each glycosyltransferase in the cps-2 gene cluster were assigned on the basis of structural homology with other Klebsiella K antigens.

Ultimo aggiornamento: 12-05-2020 - 10:33