Electron Microscopy

 

The Center for Electron Microscopy of the University of Trieste is located in the highest part of the university district, at the "Carlo e Dirce Callerio" Foundation in via Fleming 31/a and 31/b.

 

 Electron Microscopy Service Mail: microscopiaelettronica@units.it

 

Scientific Direction

Prof. Piero Giulianini        
e-mail:  giuliani@units.it
tel. +39 040 558 8715

 

Technical management and TEM responsible

Dr. Paolo Bertoncin          
e-mail: pbertoncin@units.it
cell. +39 3316990847
tel. +39 558 8890


 

  Offered Services

The scanning and transmission electron microscopy laboratory manages samples in  all the preparation and staining phases. The staff has the necessary skills to establish the most appropriate techniques for sample preparation, and to guide the most suitable choice for the observation method.

Hig Resolution digital images are obtained and subsequently interpreted together with the interested researchers, in order to obtain as much information as possible from that sample.

For users who need very prolonged microscopy sessions, an initial training is provided that allows them to use the instrument autonomously, for observation and acquisition of images.

 


 

  Available instrumentation

 

SEM

Scanning Electron Microscopy (SEM) allows to obtain three-dimensional high-resolution images from a suitable sample. The principle on which this type of microscope is based is sending a beam of primary electrons on a conductive sample and to collect the image of the investigated surface by converting the different resulting products into a signal, by means of appropriate detectors.

These signals are then reproduced on a detector whose electronic brush must be in phase with that of the SEM column. The digital image obtained has an excellent depth of field, so that the surface roughness and morphological characteristics of the analyzed sample can be seen in sharp detail. Furthermore, the X-ray analysis produced allows an accurate study of the elemental composition of the investigated material.

The SEM service is equipped with the following instrumentation:

  • Metallizer S150A Sputter Coater, whose function is to make conductive samples
  • K850 Critical Point Dryer, used in case you need to dehydrate biological samples, very delicate or fragile structure, in order to observe them in a vacuum
  • Scanning electron microscope - SEM-LEICA STEREOSCAN 430i
  • Detector for SE images (secondary electrons)
  • BSE image detector (backscattered electrons)
  • Semi-quantitative EDX microanalysis system equipped with Si (Li) PENTAFET PLUS TM detector, with ATW TM window (Oxford Instruments, United Kingdom)

 

 


 

TEM

The Transmission Electron Microscopy (TEM) allows to obtain images from a sufficiently thinn sample (<0.1 micron),  or directly from a nano-material sample.

High resolution images (0,30nm line/line) produced by high energy electrons (up to 100 KeV) is transmitted onto a fluorescent screen and then converted by ccd H.R. Camera into a large tif image file.

The TEM service is equipped with the following instrumentation:

  • Transmission electron microscope (EM 208, Philips) equipped with Quemesa (Olympus Soft Imaging Solutions) camera. Image acquisition with RADIUS software.
  • Ultramicrotome Leica Ultracut UCT8
  • Leitz Orthoplan optical microscope with condenser in light field, in dark field, in phase contrast
  • Leitz Ortholux optical microscope equipped with a camera (Leica DC 300) for image acquisition

 

                         


       

  Access to the service & user rates

 

PDF icon PDF icon Vademecum administrative accounting service microscopy

File File microscopy service request form

PDF icon PDF icon Tariff Services Microscopy 2106

 


 

  Visits, traineeships and internships

At the facility, on request, internships / traineeships can be hosted with reference to:

  •   PhD students, degree students, students from graduate schools
  •   secondary school students who carry out internships within alternanza scuola/lavoro project
  •   secondary school students for visits and demonstrations

For information contact by phone or e-mail the technical manager dr. Paolo Bertoncin

 

 

 

Last update: 10-02-2018 - 15:31