Flow cytometry & Biacore

 

Flow cytometry (FCS-DSV) and Biacore (SPR-DSV) services are available for both University of Trieste and external users.

Access to services is subject to internal guidelines (see below). Costs should be paid by the head of the research group in support of  instrument management / maintenance, in accordance to the price list  included in the guidelines.

 

  Flow cytometry (FCS-DSV)

Flow cytometry (FCS_DSV) is a technique that allows making qualitative and quantitative analysis of single cells. It is a rapid and sensitive technique capable of providing information on eukaryotic as well as prokaryotic  cells.

Biomedical and environmental applications include:

  • biomedical and environmental applications include:
  • immunophenotype determination
  • cell cycle and ploidy analysis,
  • marker analysis (membrane or cytosolic)
  • induction of apoptosis-necrosis,
  • analyses of cellular functions (phagocytosis, endocytosis, ROS),
  • binding / uptake of drugs;
  • mechanism of action of membranolitic substances against gram + bacteria and Gram-

 


 

  Surface Plasmon Resonance (SPR-DSV)

Surface Plasmon Resonance (SPR_DSV) is a high sensitivity technique that allows real-time measurements of biomolecular interactions, without requiring potentially invasive tracers. It can provide qualitative and quantitative information on the interaction selectivity, as well as on binding kinetics and binding affinity (dissociation constants).

It is applicable to the study of:

  • interactions between molecules of biological interest (proteins, nucleic acids, carbohydrates, lipids, also with small organic molecules).
  • Dissociation constants between a surface immobilized molecule (eg. protein, antibody, nucleic acid) and a molecule in the supernatant (respectively a ligand, antigen or complementary oligonucleotide/binding protein)
  • interaction between molecules (eg peptides or proteins) and model biological membranes (liposomes)

 


 

  Instruments and Contacts to access the service

 

Flow cytometry (FCS-DSV)

Instrument: Cytomics™ FC 500 (Beckman Coulter)
Person in charge: Dr. Sabrina Pacor
Accredited operators: Dr. Sabrina Pacor, Dr. Monica Benincasa

Instrument: FACScalibur (Becton Dickinson)
Person in charge: Dr. Roberta Bulla
Accredited operator: Dr. Roberta Bulla

 

Biacore SPR-DSV

Instrument: Biacore X-100 (GE)
Person in charge: Prof. Alessandro Tossi
Accredited operator: Dr. Filomena Guida

 


 

Instruments images

 

 

 


 

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Last update: 08-29-2018 - 14:20