Flow cytometry & Biacore

 

Flow cytometry (FCS_DSV) is a technique that allows making qualitative and quantitative analysis of single cells. It is a rapid and sensitive technique capable of providing information on eukaryotic as well as prokaryotic  cells.

Biomedical and environmental applications include:

  • biomedical and environmental applications include:
  • immunophenotype determination
  • cell cycle and ploidy analysis,
  • marker analysis (membrane or cytosolic)
  • induction of apoptosis-necrosis,
  • analyses of cellular functions (phagocytosis, endocytosis, ROS),
  • binding / uptake of drugs;
  • mechanism of action of membranolitic substances against gram + bacteria and Gram-

 

Surface Plasmon Resonance (SPR_DSV) is a high sensitivity technique that allows real-time measurements of biomolecular interactions, without requiring potentially invasive tracers. It can provide qualitative and quantitative information on the interaction selectivity, as well as on binding kinetics and binding affinity (dissociation constants).

It is applicable to the study of:

  • interactions between molecules of biological interest (proteins, nucleic acids, carbohydrates, lipids, also with small organic molecules).
  • Dissociation constants between a surface immobilized molecule (eg. protein, antibody, nucleic acid) and a molecule in the supernatant (respectively a ligand, antigen or complementary oligonucleotide/binding protein)
  • interaction between molecules (eg peptides or proteins) and model biological membranes (liposomes)

 

The flow cytometry (FCS_DSV) and Biacore (SPR_DSV) Service at the Department of Life Sciences provides skills and tools to Academic and external users. Access to the service is regulated and requires a contribution to support of management/maintenance costs of instruments, as specified in the rates table (see Service Rules), and is charged to the head of the research group that requires this services.

 

Contacts and instruments

 

Flow cytometry FCS-DSV

Equipments

Qualified personnel

Dr. Pacor Sabrina

pacorsab@units.it

040-558.8706

Cytomics™ FC 500 (Beckman Coulter)

Dr. Pacor S.

Dr. Benincasa M.

 

Dr. Bulla Roberta

rbulla@units.it

040-558.8654

FACScalibur (Becton Dickinson)

Dr. Bulla R.

Dr. Trevisan E.

 

 

Biacore SPR-DSV

Equipments

Qualified personnel

Prof. Tossi Alessandro

atossi@units.it

040-558.8705

Biacore X-100 (GE)

Dr. Guida F.

 


 

Selected papers

 

Effects of Two Fullerene Derivatives on Monocytes and Macrophages. Pacor S, Grillo A, Đorđević L, Zorzet S, Lucafò M, Da Ros T, Prato M, Sava G. Biomed Res Int. 2015;2015:915130. doi: 10.1155/2015/915130.

Cellular internalization and cytotoxicity of the antimicrobial proline-rich peptide Bac7(1-35) in monocytes/macrophages, and its activity against phagocytosed Salmonella typhimurium. Pelillo C, Benincasa M, Scocchi M, Gennaro R, Tossi A, Pacor S. Protein Pept Lett. 2014 Apr;21(4):382-90.

Rapid and reliable detection of antimicrobial peptide penetration into gram-negative bacteria based on fluorescence quenching. Benincasa M, Pacor S, Gennaro R, Scocchi M. Antimicrob Agents Chemother. 2009 Aug;53(8):3501-4. doi: 10.1128/AAC.01620-08. 

New aspects of the structure and mode of action of the human cathelicidin LL-37 revealed by the intrinsic probe p-cyanophenylalanine. Xhindoli D, Morgera F, Zinth U, Rizzo R, Pacor S, Tossi A. Biochem J. 2015 Feb 1;465(3):443-57. doi: 10.1042/BJ20141016.

Identification of novel proteins binding the AU-rich element of α-prothymosin mRNA through the selection of open reading frames (RIDome). Patrucco L, Peano C, Chiesa A, Guida F, Luisi I, Boria I, Mignone F, De Bellis G, Zucchelli S, Gustincich S, Santoro C, Sblattero D, Cotella D.RNA Biol. 2015;12(12):1289-300. doi: 10.1080/15476286.2015.1107702.

A single-step, sensitive flow cytofluorometric assay for the simultaneous assessment of membrane-bound and ingested Candida albicans in phagocytosing neutrophils. Busetto S, Trevisan E, Patriarca P, Menegazzi R. Cytometry A. 2004 Apr;58(2):201-6.

Mast cells kill Candida albicans in the extracellular environment but spare ingested fungi from death. Trevisan E, Vita F, Medic N, Soranzo MR, Zabucchi G, Borelli V. Inflammation. 2014 Dec;37(6):2174-89. doi: 10.1007/s10753-014-9951-9.

 


 

Instruments images

 

 

 

 

 

 

Last update: 01-27-2017 - 14:56