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DNA Sequencing Service
DNA Sequencing Service
Building Q, via Licio Giorgieri, 5 - 34127 Trieste
Department of Life Sciences, University of Trieste
The Sequencing Service takes advantage of the ION TORRENT PGM platform consisting of the Ion OneTouch™ 2 System, an automated system used for the generation of template-positive Ion Sphere™ Particles (ISPs) for semiconductor sequencing. The Ion OneTouch™ 2 System contains the Ion OneTouch™ 2 and Ion OneTouch™ ES Instruments. The new Personal Genome Machine (PGM™) perform, then, the sequencing. The sequencing technology underlying the Personal Genome Machine (PGM™) exploits a well-characterized biochemical process: When a nucleotide is incorporated into a strand of DNA by a polymerase, a hydrogen ion (H+) is released as a byproduct. This hydrogen ion carries a charge which the PGM™ System’s ion sensor-essentially the world's smallest solid-state pH meter- can detect. As the sequencer floods the chip with one nucleotide after another, any nucleotide added to a DNA template will be detected as a voltage change, and the PGM™ System will call the base. A principal component of the Personal Genome Machine™ System is the sequencing chip. This microprocessor chip incorporates an extremely dense array of >1 million micro-machined wells married to an ion sensor. Each well contains a different DNA template, allowing massively parallel sequencing.
The Sequencing Service have also at his disposal an Applied Biosystems® 3130 Genetic Analyzer, a 4-capillary electrophoresis instruments for the low to medium sequencing throughput. This instrument run a wide variety of sequencing and fragment analysis applications including microsatellite analysis, AFLP, LOH, SNP validation, and de novo sequencing and resequencing (mutational profiling). The sequencer can process to 96- or 384-well plates.
OPERATIONS AND SERVICES
The Sequencing staff puts their expertise available for sequencing projects management from primers design to DNA extraction, sequencing and data analysis both as a service or in collaboration works.
The staff has a great experience 16S, 18S metagenomics, COI analysis and in libraries preparation to study marine and terrestrial microbial and fungi community and human microbiota.
Our experience in STR DNA fragment analysis for human identification and cell lines characterization, covers several decades.
Giuseppina Campisciano, Fiorella Florian, Angela D'Eustacchio, David Stanković, Giuseppe Ricci and Manola Comar. 2017.
Subclinical Alteration of the Cervical-Vaginal Microbiome in Women with Idiopathic Infertility. Journal of Cellular Physiology. Accepted manuscript online: 18 JAN 2017, DOI: 10.1002/jcp.25806
Last update: 02-08-2017 - 11:08